The gel setup is thus:

Lane 1 -- Positive control -- this is a reamplification of the original DNA with the band that was to be isolated circled.

Lane 2 -- negative control - nice to see nothing in a lane

Lanes 3, 4, and 5 - amplification of DNA from circled band in lane 1. The difference between the lanes is that the first lane is from the original reaction tube in which the needle was plunged after being stuck into the gel. Lane 4 is a 1:2 dilution of the original. Lane 5 is a 1:4 dilution of the original.

Lane 5 is a size marker (100 bp)

I can see the band of interest in the experimental lanes, but there is lots of other DNA.